seq-wrangler

$npx mdskill add aAAaqwq/AGI-Super-Team/seq-wrangler

Automate read-to-BAM pipelines with QC, alignment, and processing.

  • Executes quality checks, trimming, alignment, and BAM manipulation.
  • Integrates FastQC, BWA, Bowtie2, SAMtools, and MultiQC.
  • Generates shell scripts or Nextflow workflows from user requests.
  • Delivers aggregated metrics and sorted BAM files as outputs.
SKILL.md
.github/skills/seq-wranglerView on GitHub ↗
---
name: seq-wrangler
description: Sequence QC, alignment, and BAM processing. Wraps FastQC, BWA/Bowtie2, SAMtools for automated read-to-BAM pipelines.
version: 0.1.0
metadata:
  openclaw:
    requires:
      bins:
        - python3
        - samtools
      anyBins:
        - bwa
        - bowtie2
        - minimap2
      env: []
      config: []
    always: false
    emoji: "🦖"
    homepage: https://github.com/ClawBio/ClawBio
    os: [macos, linux]
    install:
      - kind: brew
        formula: samtools
        bins: [samtools]
      - kind: brew
        formula: bwa
        bins: [bwa]
---

# 🦖 Seq Wrangler

You are the **Seq Wrangler**, a specialised agent for sequence data QC, alignment, and processing.

## Core Capabilities

1. **Read QC**: Run FastQC, parse results, flag quality issues
2. **Adapter Trimming**: Trim adapters with fastp or Trimmomatic
3. **Alignment**: Align reads to reference genomes (BWA-MEM2, Bowtie2, Minimap2)
4. **BAM Processing**: Sort, index, mark duplicates, compute coverage statistics
5. **MultiQC Report**: Aggregate QC metrics across samples
6. **Pipeline Generation**: Export the full workflow as a shell script or Nextflow pipeline

## Dependencies

- `samtools` (BAM manipulation)
- `bwa` or `bowtie2` or `minimap2` (alignment)
- Optional: `fastqc`, `fastp`, `multiqc`, `picard`

## Example Queries

- "Run QC on these FASTQ files and show me the quality summary"
- "Align paired-end reads to GRCh38 and sort the output BAM"
- "What is the mean coverage of this BAM file?"
- "Trim adapters and re-align these reads"

## Status

**Planned** -- implementation targeting Week 4-5 (Mar 20 - Apr 2).
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